If not available, please send an email to and request a sample submission form. Sample Submission Form Please use the sample submission form from our website for your sample submission: ( genetics/cell-line-identification), please select “Sample Submission Form”). Ideally a unique identifying number and the name of the submitting person is written onto the tube with a permanent marker, or the 96well plate is labeled with the name of the submitter and the date of submission. Labeling of samples Samples need to be labeled according to the sample submission form that is submitted electronically with the samples. More than 20 samples should be submitted in 96 well semiskirted PCR plates (Catalogue # HSS9901, BioRad). Sample tubes Less than 20 samples can be submitted in 1.5ml Eppendorf tubes (1.5ml “3810 X PCR clean”, Catalogue # 0030125.215 Eppendorf). Supernatant For Mycoplasma testing please supply 1ml of supernatant (or cells with supernatant). The DNA should have been cleaned by a column and have a concentration of >10ng/ul. Genomic DNA If DNA was extracted from cells, DNA can also be submitted. 1 to 5 million cells will give a DNA concentration after extraction of 20 to 100ng/ul. The cell pellet we need is therefore very small. Overloading the system with DNA leads to problems in peak differentiation. 1 to 2ng total genomic DNA is sufficient. Cell-line Identification Service Sample Submission GuidelinesĬells from Cell culture Cell-lines The PowerPlex18D System is sensitive to DNA input.
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